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SDS-PAGESodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis
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Electroforesis de proteinas.--La electroforesis se realizo usando un equipo de electroforesis vertical (Mini-PROTEAN, Bio-Rad Laboratories) utilizando un sistema discontinuo-denaturante (SDS-PAGE) segun Laemmli (1970).
It has been stated that the SDS-PAGE technique is very suitable for distinguishing meat proteins and for the determination of meat types in some meat mixtures and products (Ekici and Akyuz, 2003; Aslam, et al., 2018).
Aliquots were prepared and SDS-PAGE was done for isolation of whey proteins.
The bacterial lysates were analyzed by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
The results provided by the colorimetric assay on protein extracts and the electrophoretic profiling by SDS-PAGE confirmed that Tris-Cl and urea-TBS buffers could be similarly used for protein extraction from processed and unprocessed peanuts.
Total protein analysis was carried out by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) as described previously.
The SDS-PAGE analysis of protein subunits indicated that each processed product contained different proportions of protein fractions.
The concentrated EgCF proteins were separated using 10% SDS-PAGE.
Total trophozoite extract and the purified [alpha]-13 giardin fusion protein were subjected to 12% SDS-PAGE and transferred to nitrocellulose membrane.
SDS-PAGE analysis in 10% polyacrylamide gel showed the expression of the proteins of the tissues namely native bovine omasum, decellularised bovine omasum with 0.5% SDS at 48 h interval and its comparison with different biological detergents at same percentage and at 24 h interval.
Keywords: Submerged fermentation, ABTS, SDS-PAGE, Substrate, optimum production.
Protein markers (10-200 kDa) were used for SDS-PAGE. The gel images were then scanned and analyzed by Image J software.