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In vitro, SHFF are much larger, displaying round and flat appearance compared to HFF.
SA-[beta]-Gal: Senescence-associated beta-galactosidase DMEM: Dulbecco's modified Eagle's medium EDA: Extract from Deschampsia antarctica EDTA: Ethylenediaminetetraacetic acid FDG: Fluorescein di-[beta]-galactopyranoside HFF: Human foreskin fibroblasts LmnA/C: Lamin A/C MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetra- zolium bromide PCNA: Proliferating cell nuclear antigen PD: Population doubling SHFF: Senescence human foreskin fibroblasts SIPS: Stress-induced premature senescence SIPSF: Stress-induced premature senescence fibroblasts Sirt: Sirtuin.
(d) Densitogram (left panel) and Western blot (right panel) of PCNA in controls (C13, cells of 13 PD (HFF) and C45, cells of 45 PD (SHFF)), and cells exposed to 100, 150, and 200 [micro]M [H.sub.2][O.sub.2] during 2 h and after 24 h of recovery.