SISH

AcronymDefinition
SISHSelf Injury Self Help (support group; Bristol, UK)
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We classified breast cancer phenotypes according to the IHC results for ER, PR, HER-2 and Ki-67, and SISH results for HER-2 as follows [20]: luminal A type: ER or/and PR positive and HER-2 negative and Ki-67 LI <14%; luminal B type: (HER-2 negative) ER or/and PR positive and HER-2 negative and Ki-67 LI >14% and (HER-2 positive) ER or/and PR positive and HER2 overexpressed or/and amplified; HER-2 type: ER and PR negative and HER-2 overexpressed or/and amplified; and triple negative breast cancer (TNBC) type: ER, PR, and HER-2 negative.
Evaluation of automated silver-enhanced in situ hybridization (SISH) for detection of HER2 gene amplification in breast carcinoma excision and core biopsy specimens.
RNA section in situ hybridization (SISH) was carried out on 25 differentially expressed genes identified, including that of a novel gene, 3110079O15Rik, and it was ascertained that their expression domains overlapped with that of Sox9 (Table S2).
The results for HER2 IHC and SISH on biopsy, resection, or both in the 100 cases are summarized in Tables 2 and 3.
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Silver in situ hybridization (SISH) was performed on undetermined cases with an automatized system (Ventana Benchmark XT, Ventana Medical Systems, Illkirch, France) (14).
However, in a study that compared HER2 status in the metastatic lymph nodes compared to primary tumor, IHC and silver in situ hybridization (SISH) were used to compare HER2 status.
Comparison of automated silver enhanced in situ hybridisation (SISH) and fluorescence ISH (FISH) for the validation of HER2 gene status in breast carcinoma according to the guidelines of the American Society of Clinical Oncology and the College of American Pathologists.
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