SMA1

AcronymDefinition
SMA1Spinal Muscular Atrophy, Type I
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Conor has the severest form SMA1,from which 80% of victims die before they are a year old.
Sma1 has the parentage `Steptoe'/2*Diamant'/3/Minn Dwarf 64.98-8/`Briggs'/4/`Asse' (Muir and Nilan, 1973; Schaller and Prato, 1968).
Pulsed-field gel electrophoresis patterns of chromosomal Sma1 digests of all four isolates were identical.
All GAS isolates undergo pulsed-field gel electrophoresis (PFGE) with Sma1 by methods described elsewhere, with the exception that an Enterococcus isolate was used as the standard (21).
Sma1 has the parentage `Steptoe'/2*`Diamant'/3/Minn Dwarf 64.98-8/`Briggs'/4/`Asse' (Muir and Nilan, 1973; Schaller and Prato, 1968).
In 2003 in the Netherlands, a new methicillin-resistant Staphylococcus aureus (MRSA) strain emerged that could not be typed with Sma1 pulsed-field gel electrophoresis (NT-MRSA).
Sma1 has the parentage `Steptoe'/2*`Diamant'/3/Minn Dwarf 64.98-8/ `Briggs'/4/`Asse' (Muir and Nylan, 1973; Schaller and Prato, 1968).
Molecular relation of strains was determined by using pulsed-field gel electrophoresis (PFGE) after digestion of genomic DNA with restriction endonuclease Sma1 (Gibco BRL, Prmncga, Madison, WI) (13).
Genomic DNA in agarose plugs was digested with Sma1 and placed into 1% agarose gel.
Chromosomal DNA was digested with Sma1 (40 U/block); gel slices were embedded in 1% (wt/vol) PFGE agarose gels (Bio-Rad Laboratories) and separated by electrophoresis on a CHEF 3D system (Bio-Rad Laboratories) in X 0.5 Tris-Borate-EDTA buffer.