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SP8Surge Protector 8
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Finally, the oocytes were observed on a slide with a cover glass under a confocal laser scanning microscope (Leica, TCS SP8, Germany) with the fluorescence excitation and emission at 579 nm and 599 nm, respectively.
All samples were investigated under a Leica TCS SP8 confocal laser scanning microscope, using standard configuration settings.
The homologous STEC O157:H7 Sakai prophages (Sp2, Sp3, Sp6, Sp8, and Sp14) identified in the BLAST analysis were added to the image according to their known locations (Table 2).
Microscopic images were obtained using a confocal microscope (Leica TCS SP8; Leica Microsystems, Mannheim, Germany).
Fibroblast migration was monitored by live cell imaging at 37[degrees]C and 5% C[O.sub.2] using a confocal laser scanning microscope (TCS SP8, Leica Microsysteme Vertrieb GmbH, Wetzlar, Germany).
([degrees]C) OPC-S 22.0 38.5 4.9 31 BPC-S 63.5 4.8 30 FPC-S 63.0 4.4 30.5 OPC-W 23.5 42.0 4.1 8.5 BPC-W 61.5 4.2 10 FPC-W 61.2 4.2 8.5 *(1) High range water reducing agent, SP8 SX2.
The following day, cell nuclei were counterstained with DAPI (Invitrogen, Grand Island, NY, USA) and mounted with mounting medium and images were captured digitally using either the Axio Imager Apotome microscope (Zeiss, Gottingen, Germany) or the Confocal Laser Scanning microscope (Leica TCS SP8, Buffalo Grove, IL, USA).
Fixed cells were stained with DAPI for 15 minutes, washed 3 times with PBS, and observed with a confocal microscope (GFP excitation spectrum: 488 nm; DAPI excitation spectrum: 405 nm) (Leica TCS SP8, Germany).
These are the genes with gene symbols ACMSD (on chromosome 2), SP8 (chromosome 7), and PXDNL (chromosome 8).
Cell fluorescence images were obtained by confocal microscope (Leica SP8) with a magnification of 100 X.
In the above study, confocal images were captured using a Leica TCS SP8 microscope (Leica, Mannheim, Germany).
Louis, MO) and photographed by a Leica SP8 confocal microscopy system (Bio-Rad, Hercules, CA).