The Salmonella spvB virulence gene encodes an enzyme that ADP-ribosylates actin and destabilizes the cytoskeleton of eukaryotic cells.
Selected key genetic features for zoonotic source prediction of Salmonella enterica serotype Typhimurium using a Random Forest classifier Affected Feature rank * gene Feature type 1 fiiC Single-nucleotide polymorphism 5 traA Accessory gene 6 spvB Accessory gene 9 1930 ([dagger]) Accessory gene 11 1874 ([dagger]) Indel 13-15, 28 cusCFBA Accessory gene 16 silP Accessory gene 21 yafA Accessory gene 24 sspH2 Accessory gene 27 0286A ([dagger]) Accessory gene 31 pipB2 Accessory gene 32 proQ Accessory gene 34 spvD Accessory gene 37 Trap Accessory gene 39 yhfL Indel 41 0835 ([dagger]) Accessory gene 43 traJ Accessory gene 45 yceA Accessory gene 46 exc Accessory gene Feature rank * Gene function (reference) 1 Motility, serotype diversity, intestinal colonization (16) 5 Pilin precursor.
Amplification was performed in a thermocycler (MJ Research, PTC 200 USA) under the following conditions: 1 cycle of initial denaturation at 95[degrees]C for 5 min, denaturation at 94[degrees]C for 30 sec, and annealing for 1 min at 53[degrees]C for spvB, spiA, pagC, and msgA and at 56[degrees]C for sipB and spaN and 2 min at 72[degrees]C, with a final cycle of 10 min at 72[degrees]C, followed by a hold at 4[degrees]C.
In contrast, spvB was only detected in 1 out of 21 (5%) isolates from the epidemic and in 80% of the sporadic cases (Table 4).
All the virulence genes assayed except spvB were present in all the isolates belonging to serogroups A, B, C1, and C2 and in more than 80% of isolates belonging to serogroups D and E.
The msgA, spvB, and pagC genes encode products that are associated with cell invasion, survival within the cell, and adhesin or pili production [30-32].
In contrast to these highly prevalent genes, spvB gene was found in <5% and 15% of isolates from human epidemic and animal origins, respectively, whereas it occurred in about 80% of clinical isolates.
Guiney, "The Salmonella spvB virulence gene encodes an enzyme that ADP-ribosylates actin and destabilizes the cytoskeleton of eukaryotic cells," Molecular Microbiology, vol.
Caption: Figure 3: Multiplex PCR results for spvB, sipA, pagC, and msgA genes in human Salmonella isolates.
Caption: Figure 5: Multiplex PCR results for spvB, sipA, pagC, and msgA genes in animal Salmonella isolates.