SRBCSSmall Round Blue Cell Sarcomas
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Mohiti-Asli and Ghanaatparast-Rashti [40] reported that dietary supplementation with 300 ppm of oregano essential oil (OEO) improved the European production efficiency factor in broilers, which was calculated according to body weight, livability, FCR and trial duration; 500 ppm OEO had no positive effect, while 300 ppm OEO in the diet produced higher secondary total antibody titer against SRBCs; in addition, the immunoglobulin G (IgG) titer was higher than it was in the control group.
SRBCs were washed three times with PBS.In vitro phagocytosis of SRBCs was performed in colchicine treated groups, negative and positive control groups by macrophages (Javeed et al., 2011; Loike et al.,2004; Seyrantepe et al.,2010).
Antibody response to sheep red blood cells (SRBCs): In control (PBS) and administered (vitamin-E; beta-glucans) chickens, humoral immune response was detected by haemagglutination test in terms of antibody titers (geomean titers; GMT) to sheep RBCs on day 7th and 14th after first and second injections of SRBCs.
Immunization of Rabbits: 1ml of SRBCs suspension was inoculated into marginal ear vein of each rabbit in treated and control groups on 14th day of trial(Tilwariet al., 2011).
The effect of test extract and standard drug on the DTH response in Wistar rats using SRBCs as antigen, administration of aqueous extract of CP at the dose of 400 mg/kg and 800 mg/kg and levamisole 50 mg/kg treatments which were given orally for 14 days showed significant increase in paw edema compared to control group.
Overall mean engulfment/cell was significantly (p=0.005) more after 90 min of co-incubation with SRBCs (Fig.
Antibody titers detected by microplate haemagglutination assay revealed that oral administration of EOT and CTA resulted in higher total Igs, IgG, and IgM geomean titers (GMT) against SRBCs on days 7 and 14 after primary injection (PPI) of SRBCs as compared to control group.
The results are shown in Figure 1(b); in comparison with the normal control, the generation of mouse anti-SRBC antibody (Ab) in model group was significantly increased, which suggests that the sensitization caused by SRBC has been made successfully.
Initial experiments revealed that when mice were fed with CTB-conjugated SRBC, there was an abrogation in the delayed-type hypersensitivity and IgE antibody responses upon systemic immunization with SRBCs (62).
Treatment with Sulforaphane along with the antigen, sheep red blood cells (SRBC), produced an enhancement in the circulating antibody titre and the number of plaque forming cells (PFC) in the spleen.
Every mouse was immunized with 0.5108 sheep red blood cells (SRBCs) intraperitoneally at 14th and 21st day of experiment, including positive and negative control groups.
Three in-vivo tests in which cutaneous response to phytohemagglutinin-P (PHA-P) and serum antibody level produced in response to Newcastle disease vaccine and sheep red blood cells (SRBCs) were measured to evaluate the immune competence in broiler chickens treated with SB.