Tweaking the components of the cycle again, they engineered cells to keep SREBP
in the ER while allowing SCAP to move to the Golgi on its own.
Technology: Inhibition of SREBP
by Betulin Improves Hyperlipidemia and Insulin Resistance and Reduces Atherosclerotic Plaques
Novel putative SREBP
and LXR target genes identified by microarray analysis in liver of cholesterol-fed mice.
After that, various transcription factors like E2F, YY1, NF[kappa]B, SREBP
, Smad7, etc.
Till now, three kinds of SREBPs
were identified including SREBP
-1c and SREBP
Overexpression of lnsig-1 in the livers of transgenic mice inhibits SREBP
processing and reduces insulin-stimulated lipogenesis.
1038/nature06179), Alnylam scientists and collaborators demonstrated the ability of synthetic siRNAs to silence three distinct hepatocyte-expressed genes -- apolipoprotein B, factor VII, and SREBP
cleavage-activating protein, or "SCAP" -- to levels of greater than 80 percent with both single dose and repeat dose administration of target-specific siRNAs in mice or hamsters.
2] Nonstandard abbreviations: FFA, free fatty acids; DNL, de novo lipogenesis; SREBP
, sterol regulatory element binding protein; CoA, coenzyme A; SCD1, stearoyl-CoA desaturase-1; TG, triacylglycerides; DG, diacylglycerols; CE, cholesterol ester; PL, phospolipid.
The response to the reduction of intracellular sterol is mediated by transcription factor SREBP
-1c, a member of the SREBP
-1a,-1c, and -2) plays an important role associated with fatty acid synthesis in adipose tissue.
The researchers focused on two key regulators of lipid (fat) metabolism that are found in many human cells as well: SREBP
(sterol regulatory element-binding protein) that stimulates synthesis of fatty acids in the liver, and AMPK (adenosine monophosphate kinase) that turns SREBP
on and off.
cleavage- activating protein (SCAP) is required for increased lipid synthesis in liver induced by cholesterol deprivation and insulin elevation.