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Distilled-water-solute ammonium sulfate [(NH4)2SO4] was labeled with Nenriched to 10.11 atom% 15N (SRICI Shanghai China) and supplied at rates of 0 (control) 30 (F30) 105 (F105) and 315 (F315) mg 15N seedling-1 delivered as two solution applications on 25 July and 1 August 2010.