A molecular technique based on the polymerase chain reaction (PCR), the simple sequence repeats--polymerase chain reaction SSR-PCR, is being widely used in applied entomology (Harry et al.
Genetic variability of Brazilian toxoplasma gondii strains detected by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) and simple sequence repeat anchored-PCR (SSR-PCR).
With considering the importance of this gene in fertility and ovulation rate, continuous study and research of this gene and other candidate genes, the use of techniques such as PCR-RFLP, SSR-PCR or PCR-SSCP look essential.