STAT-1 expression level was significantly increased in group 1 compared to the other two groups (p<0.05).
Activated STAT-1 was more detectable in peripheral blood mononuclear cells (PBMCs) of group 1a patients compared to group 1b patients, but the result was not statistically significant (p=0.062).
However, regarding IFNAR-2 mRNA and STAT-1 protein expressions, no difference was observed between groups 2a and 2b (p=0.590 and p=0.519) (Table-3).
IFN- receptors and STAT-1 proteins are critical components of the cellular anti-viral and anti-proliferative responses induced by IFNs.
Keywords: Co-infection, HBV, HCV, Interferon, STAT-1.
Signal transducer and activator of transcription (STAT-1) plays an important role in molecular signaling pathway.
As the magnitude of problem of co-infection is greater therefore this study has been designed to detect STAT-1 in blood of patients co-infected with hepatitis B and C to measure the influence of absence of STAT-1 in patients having interferon resistance.
They are STAT-1, STAT 2, STAT3, STAT 4, STAT5a, STAT5b and STAT 6.
Keywords: Blood expression, HCV, Interferon resistance, Predictive factor, STAT-1.
In order to understand the role of cellular factors that are important in interferon signaling cascade in blood of interferon resistance, a study was designed to detect STAT-1, an important cellular factor, in interferon resistant HCV patients.
Based on previously available sequence on NCBI, primers specific to STAT-1 was designed by using different bioinformatics tools i.e., Primer 3, E-PCR and Oligo calc.
Results showed that most of the HCV infected patients resistant to standard interferon treatment (i.e., 60%) didn't express the STAT-1 in their blood whereas all responders expressed STAT-1 (as shown in table 1).