The NIH eventually identified a de novo STIM1
(c.343A>T) mutation responsible for a gain of function of the STIM1
protein and a CRAC channelopathy .
This pathway is activated through the consorted action of two essential molecules: Orai1, a highly selective [Ca.sup.2+] channel at the plasma membrane, and STIM1
, a single pass ER membrane protein [36, 37].
Roos et al., "STIM1
is a [Ca.sup.2+] sensor that activates CRAC channels and migrates from the [Ca.sup.2+] store to the plasma membrane," Nature, vol.
Wu et al., "Suppression of STIM1
in the early stage after global ischemia attenuates the injury of delayed neuronal death by inhibiting store-operated calcium entry-induced apoptosis in rats," NeuroReport, vol.
The results in Table 3 show that indicators STIM1
(behaviors) and STIM6 (environment) display significant coefficients in both subsamples.
and orai1 mediate crac currents and store-operated calcium entry important for endothelial cell proliferation," Circulation Research, vol.
The Feinberg team, led by Murali Prakriya, assistant professor of molecular pharmacology and biological chemistry, discovered that STIM1
not only opens these pores but is responsible for determining the exquisite selectivity for calcium ions within the CRAC channels, a critical factor in kick starting the body's immune system.
Dominant-negative isoforms of STIM1
and ORAI1, -2 and -3 were provided by L.
Bochet et al., "STIM1
juxtaposes ER to phagosomes, generating [Ca.sup.2+] hotspots that boost phagocytosis," Current Biology, vol.
The cDNAs encoding human Orai-1 (hOrai-1) and human STIM1
(hSTIM1) were purchased from OriGene Technologies (Rockville, MD, USA) and then subcloned into pcDNA3.1 according to the manufacturer's protocol (Life Technologies).
(stromal interaction molecule 1) and STIM2 (stromal interaction molecule 2) have been identified as the only protein family that functions simultaneously as an intracellular calcium storage sensor and as a regulator of calcium influx from the extracellular environment.
Interesting observations were also reported with regard to Stim1
gene and the response to stress in SHRSP .