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Thus, a strong interest was generated by the stromal vascular fraction (SVF), the noncultured fraction of MSCs, directly obtained after collagenase treatment of adipose tissue .
In order to deeply investigate the basic biological mechanisms beneath the recorded clinical performances of such a graft and to investigate the bone-regenerative potential of ASCs and SVF, we studied their ability to colonize SB and generate new tissue when cultured on it .
Mesenchymal cell surface markers were analysed by flow cytometry on fresh SVF and on cultured ASCs.
The results of the study conclude that IntelliCells manufactured by IntelliCell's proprietary technology yield an average of 10 times (10X) the number of SVF cells containing adult adipose stem cells from less fat (only 2 oz) than that which the Company believes is used by any of its present competition utilizing enzymes in their process.
"We believe that the body stores all of the reparative and regenerative cells it could possibly need as a result of trauma or illness in the stromal vascular fraction (SVF) contained in the fat.
Stanevicius said the SVFS could approach non-governmental consumer protection groups if the situation remains unchanged.
Patients will be followed for 12 months after receiving a single intravenous SVF infusion.
In the lipoaspiration procedure, 100-240 ml of lipoaspirate will be extracted, and SVF was isolated with minimal manipulation and reconstituted in saline solution for intravenous administration.
Even with these phenotypic differences, tissue repair was possible using freshly isolated SVF cells .
SVF from adipose tissue consists of mainly endothelial cells, hematopoietic cells, and ASCs.
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