Double stranded PCR product was detected by fluorescent dye associated with SYBR
green-I at each extension step.
Among the 66 PBMC samples positive for BKV DNA with semi-nested PCR, viral load ranged from 146 X [10.sup.0] to 34 X [10.sup.3] copies/[10.sup.6] PBMC by SYBR
green real-time PCR.
Artificially infected blood samples were used in order to compare the in-house Line Probe Assay and SYBR
Green Real-Time PCR concerning the detection of periodontal pathogens.
pEGFP-N1 plasmid (500 ng) was incubated for one hour with SYBR
Figure 2(a) shows separation of amplification products on agarose gel after electrophoresis, and Figure 2(b) shows LAMP products in reaction tubes after staining with SYBR
Green I dye.
Using tissue homogenates of the skin and tongue mucosa, brain, lungs, liver, spleen, heart, mesenteric lymph nodes, and intestines of the 12 dead animals (a total of 70 samples), we conducted an additional SYBR
Green realtime PCR to confirm the presence of orthopoxvirus in all affected animals (Table 1).
Back in 1995, we were working with SYBR
Green I as a gel stain, and against manufacturer's recommendations tried various concentrations in real-time PCR.
Estos cebadores se evaluaron en pruebas de RT-qPCR utilizando el sistema SYBR
Green I, a partir de ARN total extraido con el kit GeneJET Plant RNA Purification (Thenno) en las 12 muestras asintomaticas y en las cuatro sintomaticas.
Virus and prokaryote enumeration from planktonic aquatic environments by epifluorescence microscopy with SYBR
: SybrGreen, Taq: hydrolysis probe, Plexor: Plexor (Promega, USA).
In this study, the enhanced sensitivity and specificity of qPCR was exploited for rapid detection and quantitation of TYLCV-OM using SYBR
China), 1 [micro]l of SYBR
Green I (diluted to 1:10 with 6xloading buffer) (Invitrogen, Australia), 1 [micro]l of the hydroxynaphthol blue dye (HNB) (3 mM, Lemongreen, Shanghai, China), 1 [micro]l of phenol red (diluted to 1:10 with 6xloading buffer) (Sigma-Aldrich Chemie GmbH) or 1 [micro]l of the GelRed[TM] dye (Biotum, India) were separately added from a stock solution to the master mix.