SYCP3Synaptonemal Complex Protein 3
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After blocking in Tris-buffered saline with Tween (TBST) containing 5% nonfat dry milk for 1 h, the membranes were incubated with polyclonal primary antibody SYCP3 (1:100,000, Abnova, Taipei, China) overnight at 4[degrees]C.
The expression of SYCP3 was detected to assess the effect of SKRBT on meiosis.
In previous studies, testosterone has been proven to be critical for the completion of meiosis and spermatogenesis in rodents.[sup][34] To determine whether the administration of SKRBT affected spermatogenesis, we thereby detected the expression of a meiotic marker, SYCP3, by immunofluorescence staining.
[41] made the next step and found SYCP1 and SYCP3 orthologs in the proteomes of various metazoan taxa, including Placozoa, Porifera, and Coelenterata.
SYCP2 and SYCP3 homologs of Crassostrea gigas (maximal scores 80 and 199, resp.) are annotated in the NCBI database.
The hypothesis is supported by the fact that proteins similar to SYCP1 and SYCP3 are found in the proteomes of basic Metazoa [41] and are absent from fungi and plants.
Azoospermia in patients heterozygous for a mutation in SYCP3. Lancet 2003; 362 : 1714-9.