SYPRSouthern Yucatan Peninsular Region (Worcester, MA)
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DNA samples were amplified in a total of 25 [micro]l of the following reaction mixture: 12.5 [micro]l QuantiTect SYPR Green (2X), 0.5 [micro]l of each primer (50 pmol), 7 [micro]l template DNA and 4.5 [micro]l water, nuclease-free.
Real-time PCR was performed in LightCycler (Roche) using Absolute [TM] QPCR SYPR Green Capillary Mix kit (Abgene), using the following gene-specific primers:
(NASDAQ: SYPR), to create a 15-course customized cyber security training program to be integrated into the Sypris Cyber Range.