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Basic selective plane illumination microscopy (SPIM) (as introduced in this review thus far) is capable of acquiring high-speed movies in a single plane of the sample.
Selective plane illumination microscopy (SPIM) was used in the study of the formation of a complex network of blood vessels, the sub-intestinal vein complex (SIV), in zebrafish over several days (Lenard et al.
Multidirectional selective plane illumination microscopy (mSPIM) (Huisken and Stainier.
In conventional dual-view inverted selective plane illumination microscopy (diSPIM), a high-speed, micro-electro-mechanical system (MEMS) mirror moves the excitation sheet through a sample, while a piezoelectric collar synchronously moves the detection objective lens to keep the focal plane coincident with the illuminated plane (Kumar et al.
While stage- and slit-scanning are fully compatible with existing dual-view inverted selective plane illumination microscopy (diSPIM) systems, they are not applicable to all samples.
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