8], 10% glycerol, 2% sodium dodecyl sulphate
[SDS], 5% 2-mercaptoethanol, 0.
The maximum relative activity (91%) was observed when 10mM sodium dodecyl sulphate
pre-incubated at 37degC for 30 min.
The effect of additives like magnesium sulphate, manganese sulphate, calcium chloride, copper sulphate, zinc sulphate, ferric sulphate, tween-80, tween-20, polyethylene glycol, carboxy methyl cellulose, sodium dodecyl sulphate
, magnesium chloride and potassium chloride was investigated (Figure-4).
1967) and Weber and Osborne, (1969) have shown that the separation of proteins by polyacrylamide gel electrophoresis in the presence of anionic detergents sodium dodecyl sulphate is dependent on the molecular weight of the polypeptide chains.
Molecular weght estimation of polypeptide chains by electrophoresis in sodium dodecyl sulphate polyacrylamide gel electrophoresis.
n] Na-cholate 3[alpha],7[alpha],12[alpha]-Trihydroxy-5[beta]-cholan- 24-oic acid sodium salt Na-deoxy- 3[alpha],12[alpha]-Dihydroxy-5[beta]-cholan-24-oic cholate acid sodium salt SDS Sodium dodecyl sulphate
SM-1200 Sucrose monolaurate Sulfobetaine N-dodecyl-N,N-dimethyl-3- SB-12 ammonio-l-propanesulphonate Thesit [Dodecylpoly(ethylene-glycolether).
Sodium dodecyl sulphate
polyacylamide gel electrophoreses (SDS-PAGE) a molecular technique is used to identify proteins related to virulence and phenotypic diversity of different strains of MG (Papazisi et al.
Effects of sodium dodecyl sulphate
, dye concentration and paraprotein on Coomassie Blue dye-binding assays for protein in urine.
ie the laboratory consumables contract has been divided into 9 lots as follows: lot 1 microbiology lot 2 molecular biology lot 3 sodium dodecyl sulphate
polyacrylamide gel electrophoresis (sds page) lot 4 plastic ware lot 5 tissue culture lot 6 general laboratory consumables lot 7 chemicals lot 8 glassware lot 9 kits
Polypeptide analysis of purified hydatid cyst fluid (HCF) from ruminants (sheep, goat, cattle, buffalo and camel) and human beings was performed by sodium dodecyl sulphate
polyacrylamide gel (12%) electrophoresis (SDS-PAGE) under reduced conditions.
Sodium dodecyl sulphate
electrophoresis of urinary proteins.
Polyacrylamide gel electrophoresis in sodium dodecyl sulphate
containing buffers using multiphasic buffer systems: properties of the stack, valid RF measurements and optimized procedure.