[112.] Dobrydneva Y, Blackmore P (2001) 2-Aminoethoxydiphenyl borate directly inhibits store-operated calcium entry
channels in human platelets.
Caption: Figure 6: Changes of the cytosolic [Ca.sup.2+] level of platelet groups in relation to activation by thiomersal in [Ca.sup.2+]-free buffer and the consequent store-operated calcium entry
. Thiomersal evoked a prolonged elevation of cytosolic calcium in all the platelets followed by sustained storeoperated calcium entry (a).
Abbreviations SOCE: Store-operated calcium entry
[[[Ca.sup.2+]].sub.i]: Intracellular [Ca.sup.2+] concentration SOC: Store-operated channel STIM1: Stromal interaction molecule 1 STIM2: Stromal interaction molecule 2 UDP: Uridine diphosphate ATP: Adenosine triphosphate LPS: Lipopolysaccharide CPA: Cyclopiazonic acid TG: Thapsigargin TNF-[alpha]: Tumour necrosis factor-a IL-6: Interleukin-6 siRNA: Small interfering RNA WT: Wild-type MB: Methylene blue TLR4: Toll-like receptor 4 NFAT: Nuclear factor of activated T cells ER: Endoplasmic reticulum.
Import occurs at the level of (i) cell plasma membrane through the calcium release-activated [Ca.sup.2+] channel protein 1 (ORAI1), the store-operated calcium entry
channels (SOCE), and specific receptor-operated channels (ROC) such as AMPA, NMDA, TRPC, and the voltage-dependent calcium channels (VDCC); (ii) endoplasmic reticulum (ER) through the sarco/endoplasmic reticulum calcium ATPase (SERCA); (iii) mitochondria intermembrane space through the voltage-dependent anion channel (VDAC); and (v) in the matrix by the mitochondrial uniporter (MCU), in synergy with the mitochondrial calcium uptake (MICU) system.