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To stain DNA for cell-cycle analysis with flow cytometry, 4',6-diamidino-2-phenylindole (DAPI), a fluorescent DNA stain, was used at a concentration of 10 pg [mL.sup.-1], and nRNA was stained with Invitrogen Syto RNASelect (1) green fluorecent cell stain (S32703, Life Technologies Corp., Carlsbad, CA), hereafter referred to as Syto RNASelect stain.
Viable bacteria counting methods (i) CFU plate counting (ii) Radiolabelling (iii) CTC staining (iv) Resazurin assay (v) FDA assay Microscopy for counting and morphological observation methods (i) Light microscopy (ii) Image-analyzed epifluorescence (iii) SEM (iv) CLSM (v) AFM (vi) FTIR Other direct and indirect method (i) Spectrophotometry (ii) Coulter count (iii) Biochemical markers Molecular biological techniques Determination of bacterial surface characteristics Contact angle measurements Methods of evaluating biofilms Morphology: (i) Image-analyzed epifluorescence (ii) SEM (iii) CLSM (iv) AFM Biofilm content: (i) Crystal violet assay (ii) Syto 9 (iii) Resazurin (iv) FDA assay TABLE 2: Techniques used in determining antibacterial activity of materials.
When the treatment ended, coverslips were rinsed three times with PBS and stained with SYTO 9 and propidium iodide from the LIVE/DEAD BacLight Bacterial Viability Kit according to the manufacturer's instructions (Invitrogen Molecular Probes, USA); the coverslips were then examined using confocal laser scanning microscopy (CLSM) [29].
The LIVE/DEAD BacLight Bacterial Viability Kit (Invitrogen, Molecular probes, Darmstadt, Germany) adopts two stains--green fluorescent SYTO 9 stain (component A) and red-fluorescent propidium iodide stain (component B) [20].
PCR for HRMA analysis was performed in 0.1-mL tubes on a rotor-based platform, Rotor-Gene 6000 (Corbett Life Science) in the presence of the fluorescent DNA intercalating dye, SYTO 9 (Invitrogen) as previously described (32).
nucleatuin (slender rods with tapered ends), all cell types could be visualized within the biofllm by staining with the nucleic acid marker Syto 59 (Molecular Probes, Eugene, OR).
Bacterial cells were Labeled green (SYTO 9), EPS was labeled red(Alexa Fluor647), and red and green super imposed appear as yellow.
Why speak of Haitian theatre (not officially part of the French Antilles, something McKay does not specify) without referring to Franketienne, Syto Cave, Herve Denis, or la Compagnie Kouidor, all of whom have greatly influenced the theatre of Guadeloupe and Martinique?
El contaje bacteriano se llevo a cabo a traves de las tecnicas de dilucion seriada y contaj e directo de celulas vivas y muertas por microscopia de epifluorescencia (con los fluorocromos Syto 9 y Yoduro de Propidio), antes de cada ensayo y a las 24 horas de exposicion, tanto a nivel planctonico como sesfl.
The researchers stained the biofilms for CSLM using SYTO 9 and propidium iodide.
Some of the most important fluorophores on the market for live cell imaging now include Cy7, Alexa 750, and new nuclear dyes including SYTO 63 and TO-PRO-5.
The green fluorescent stain (SYTO 9, with excitation/emission maxima of about 480/500 nm) colors healthy bacteria that have intact membranes, thus staining live cells; and the red dye (propidium iodide with excitation/emission maxima of about 490/635 nm) colors bacteria with damaged membranes, by displacing the green dye, thus staining dead cells.