Repertoire Analysis of Genes Encoding T3SS
Effectors and Plasmid-Encoded Virulence Factors
We further determined whether T3SS
was necessary for this effect and the role of Rho GTPases.
The amino acid sequence of VopT revealed 45% and 44% identity with the C-terminal ADP-ribosyltransferase (ADPRT) domain of two effector proteins secreted by T3SS
is found in a large number of gram-negative pathogens and symbionts .
amylovora has a type III secretory system (T3SS
) that delivers effector proteins into host plants (Lindgren et al., 1986; Kim and Beer, 2000).
In this study, we expressed the T3SS
needle protein of C.
(2) manifestaron que la activacion del inflamasoma NLRC4 requeria de la presencia simultanea de un sistema de secrecion intacto tipo III (T3SS
) o tipo IV (T4SS) necesario para el ingreso de los factores de virulencia bacterianos y de pequenas cantidades de flagelina; en particular se ha demostrado que es una region en el extremo C-terminal de flagelina la que activa el inflamasoma a traves del receptor NLRC4 (51).
Salmonellae encode a type III secretion system (T3SS
) within Salmonella pathogenicity island 1 (the SPI-1 T3SS
), which is required for bacteria-mediated endocytosis and intestinal epithelial invasion.
Intestinal epithelial cells are normally nonphagocytic; however, Salmonella have the capacity to invade these cells through a sophisticated machinery termed a type 3 secretion system (T3SS
); the genes encoding the T3SS
are located on Salmonella pathogenicity island 1 (SPI-1) [36, 37].
Of these, the best studied is the type III secretory system (T3SS
), which enables bacterial pathogens to deliver effector proteins into eukaryotic cells .
When the bug encounters low oxygen conditions, a mechanism called the Type III Secretion System (T3SS
) is triggered.
Both strains were negative for potential virulence genes, with the exception of the marine strain, which was carrying the T3SS
(type III secretion system) gene cluster, which codes for an important virulence factor used by several Gram-negative bacteria to deliver effect or proteins to host cells.