By this way, unreacted chemicals washed out from the gel, and only pyranine and TAAB
molecules bound to the polymer strands chemically, were stayed in the gel.
2-Hydroxyethyl-methacrylate Technovit 7100 was obtained from Kulzer (Wehrheim, Germany) and TAAB
812 resin from TAAB
(Aldermaston, Berks, England).
A graded ethanol series was used to dehydrate fixed specimens, which were then transferred through three changes of propylene oxide and infiltrated and embedded in TAAB
812 epoxy resin (Marivac, Halifax, NS, Canada).
In samples destined for transmission electron microscopy, ethanol was replaced with propylene oxide and then the eggs were transferred through infiltration media consisting of mixtures of propylene oxide and TAAB
812/Araldite CY212 resin (75:25, 50:50, 25:75) for 2 h in each mixture in capped vials (after Cross, 1989).