TAHV has [approximately equal to] 72.5%-84.7% nucleotide identity with LACV and SSHV and 69.2%-80.3% with JCV and INKV (30).
Certain strains of TAHV, but not JCV, have been shown to cause neurologic disease in weanling Swiss Webster mice after intraperitoneal inoculation, although both viruses were capable of replicating in the brain after intracranial inoculation (40,41).
Determining how LACV, SSHV, TAHV, JCV, and INKV differ in pathogenesis in animal and cell culture models could help explain their differing disease outcomes in humans.
To ensure that diagnostic criteria of acute infection were met, as previously described, IgM-positive samples were confirmed with IgG IFA testing (5) and studied with INKV prototype strain KN3641 (1), CHATV Mohko strain M07-1 (13), and TAHV prototype strain Bardos 92 (28) plaque-reduction neutralization test (PRNT) (13).
In that study, TAHV and undefined California serogroup infections (i.e., neutralization tests found no difference between INKV and TAHV) occurred more frequently than INKV (10).
coli urosepsis) 14 Neg MS disease, Hospitalized, acute hypothyroidism infection (HSV infection) 15 Neg - Hospitalized, acute infection (impetigo contagiosa) 16 ND - Hospitalized, multiple infarcts in the central nervous system 17 Neg - Hospitalized, epidemic nephropathy PRNT Titer Patient INKV CHATV TAHV no.
The sequence amplified by the bunyavirus genus primer had a high homology with TAHV. Subsequently, microplate plaque-reduction neutralization tests (14) were performed with BHK-21 cells and immune ascites fluid with immunity to prototype TAHV (Bardos 92; provided by the Centers for Disease Control and Prevention (CDC), Fort Collins, CO, USA) to validate the molecular identification.
Phylogenetic analyses of the nucleotide sequences of the S (Figure 2, panel A) and M (Figure 2, panel B) segments generated highly comparable topologies, which indicates that XJ0625 has a high level of sequence homology with TAHV. To provide independent confirmation, we sent the XJ0625 viral RNA to CDC, Fort Collins, Colorado, USA, for further characterization.
To determine whether persons in the region were becoming infected with TAHV, we collected serum samples from 323 persons who visited an outpatient clinic in Jiashi County and its adjacent counties in Kashi from August 18 through September 20, 2007.
Against TAHV, 82 (16.5%) of 497 study participants had neutralizing antibodies, and 74 (14.9%) were seropositive in HIT.
Seroconversion ([greater than or equal to] 4-fold rise in titer) was found with TAHV only.
In general, clinical symptoms of TAHV infection are milder in adults than in children (7).