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The PM steaks demonstrated greater (p<0.05) TBARS than LL counterparts on days 5 and 9 (Table 1), whereas the lipid oxidation was not detected in both muscles on day 0.
The mean values of the oxidative stress marker in the form of the total lipid peroxides level (TBARS [|M/l]), as determined in the plasma of the experimental and control subjects, amounted to 2.25 [+ or -] 0.60 and 1.19 [+ or -] 0.26, respectively.
The resultant pink colour was the index of TBARS. Plasma TBARS was stated as nmol/ml.
TBARS are also a by-product of the damage that results from oxidative stress.
To calibrate the TBARS concentration, the molar extinction coefficient of 154 mM-1cm-1 was used (Lapenna and collaborators, 2001).
Thiobarbituric acid-reacting substances (TBARS) were measured as the quantity of malondialdehyde-TBA 1:2 adducts spectrophotometrically at 535 nm (14).
Measurement of TBARS and antioxidant enzyme activities
TBARS concentrations were increased as expected in PG but the difference between the groups was not significant (p>0.05).
As an index of lipid peroxidation, the yielding of thiobarbituric acid reactive substances (TBARS) during an acid-heating reaction was determined.
Color, TBARS and cooked yield were minimally affected by the other ingredients.
The pH and color were measured on the remaining sample which was then frozen (and vacuum packaged) for TBARS (Thiobarbituric Acid Reactive Substances) and moistures analysis.
The objective of this work was to characterize the biomarkers TBARS and CAT in Aegla singularis Ringuelett (1948), aiming the future application of this methodology in the biomonitoring programs of different aquatic ecosystems in which the species be widespread.
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