PON1 lactonase activity in the culture's supernatant was measured as the hydrolysis of 5-thiobutyl butyrolactone (TBBL), as described [16].
Abbreviations DTNB: 5,5,-Dithio-bis-2-nitrobenzoic acid GADPH: Glyceraldehyde-3-phosphate dehydrogenase GC-MS: Gas chromatography-mass spectrometry HDL: High-density lipoproteins HUVEC: Human umbilical vein endothelial cells LDL: Low-density lipoproteins PARP: Poly (ADP-ribose) polymerase PON1: Paraoxonase-1 TBBL: 5-thiobutyl butyrolactone UPLC-MS/MS: Ultrahigh performance liquid chromatography-tandem mass spectrometry.