After cooling down, GMA (4 mmol), TBPB
(0.4 mmol), and BHT (0.04 mmol) were added and stirred at 90 [degrees]C for another 3 h (Figure 4).
The main goal of the present work paper is the investigation of mixed dye films of two dyes TBPB
and DCP to be a dosimeter for food irradiation.
NG-MAST showed one isolate with por allele 1808 and tbpB
allele 29, which is sequence type 2992.
Then, precalculated amount of initiator, TBPB
was added into the mixture.
In this work TBPB
seemed to be a suitable peroxide because complete decomposition of the peroxide as well as sufficient reaction time during extrusion could be ensured.
NG-MAST was performed as described (12) and allele numbers of porB and tbpB
genes and sequence types (STs) were assigned by using the NG-MAST website (http://www.ng-mast.net).
Styrene, tert-butyl peroxybenzoate (TBPB
), and melamine were obtained from Sigma--Aldrich (St.
The NG-MAST strategy uses 2 genes, por and tbpB
, for porin and a transferrin-binding protein, respectively.
At the end of the prepolymerization mass stage, 1 ml of ter-butyl perbenzoate (TBPB
) was added to the reaction mixture to be used as an initiator in the termination stage.
The 2.1-kb tbpB
gene was amplified by using an internal primer located near the ATG start codon (3'-Met2) and a primer located in the intergenic region between tbpB
and tbpA (5'-Inter1), as described (16).
The HIPS were prepared by a bulk-suspension process as follows: in 1 gallon capacity stainless steel reactor, with an anchor-turbine stirrer and at room temperature: (i) a fixed amount (8 wt%) of graded block copolymer with respect to monomer was dissolved into styrene at a stirring rate of 20 rpm until the total dissolution of the copolymer; (ii) benzoyl peroxide (BPO) was added, and a stirred bulk prepolymerization stage at 40 rpm and 80[degrees]C took place until the monomer conversion was close to 30-35%; (iii) during the finishing stage 0.1 wt% of ter-butyl perbenzoate (TBPB
) and a suspension medium were added, and the polymerization was carried out to total monomer conversion.
For example, in Moraxella catarrhalis, the recombinant transferrin [ILLEGIBLE TEXT] protein B (TbpB
) has been shown to elicit bactericidal antibodies (41) In N.