TBSTTris Buffered Saline with Tween
TBSTTotal Business Services Training (Australia)
TBSTTanzania Book Support Trust (est. 1999)
TBSTTony Backhurst Scuba Travel (UK)
References in periodicals archive ?
After being washed three times with TBST buffer, each membrane was incubated for 1 hour at room temperature with an alkaline phosphatase-conjugated goat anti-mouse secondary antibody (Novus Biologicals, LLC, Littleton, CO, USA) at a dilution of 1 : 5000.
After being washed with TBST, the membranes were incubated with proper secondary antibodies (Beyotime, Shanghai, China).
Secondary antibody was added after three times of washing with TBST buffer and then it was incubated for 1 hour.
Then strips were incubated with goat anti-mouse IgG Fc specific conjugated to horseradish peroxidase (HP) (1:5000) for 1 h at room temperature followed by vigorous washing with TBST and addition of chemi-luminescent substrate (Vector Lab) for HP.
The goat antirabbit IgG peroxidase conjugate (category code BA1054), prestained protein molecular weight markers (category code ARI 113), N-N'-methylene-bis-acrylamide (category code AR1161), PMSF (AR1178), sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protein loading buffer (AR1112), TBS (AR0193), TBST (AR0195), phosphate-buffered saline (PBS; 10 mM, pH 7.
4) and blocked with 5% milk (or 5% casein) in TBST for 2 h at room temperature, followed by incubation with detection antibodies (40 [micro]L of detection CUZD1 antibody from the USCN ELISA kit andl:8 dilution of CA125 antibody from R&D Systems).
A mixture containing TRITC labeled anti-PML monoclonal antibody PG-M3 and 1X TBST at a 1:10 ratio (100 [micro]L) was used for immunofluorescence staining for PML proteins on the feathered edge of each smear.
After 4 washes in TBST, the membranes were incubated with a secondary antibody (Santa Cruz Biotechnology, Inc.
Primary antibody dilution was prepared in TBST containing 1% BSA and incubation was carried out at 4[degrees]C for 12hrs with shaking at intervals.
The PVDF membrane was subsequently soaked in TBST (TrisBuffered Saline and Tween 20) containing 5% skim milk powder and put on a shaker at room temperature for 2 hours.
Para a imunodeteccao, a membrana era bloqueada com tampao (pH 7,6) Tris HCL 0,02M, NaCl 0,15M, Twen 0,05% (TBST), contendo 5% de leite em po desnatado, por 12 horas em agitacao suave e, em seguida, lavada 3 vezes com TBST (10 min cada lavagem) e incubada "overnight" com anticorpo policlonal contra HSP70 (SPA-810, StressGen, Canada), diluicao 1:1000 em TBST.