TCCCATottori Center for Climate Change Actions (Japan)
TCCCAToronto Congenital Cardiac Centre for Adults (Canada)
TCCCATokushima Center for Climate Change Actions (Japan)
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The RT-PCR was performed immediately after RNA isolation by using the specific primer pair for CoV, Cor-FW 5' [right arrow] 3' (DNA) ACTCAAATGAATTTGAAATATGC, and Cor-RV 5' [right arrow] 3' (DNA) TCACACTTTGGATAA TCCCA that amplifies a 251-bp fragment of the polymerase gene (11).