Table 2 shows that 13/37 (35.1%) carried both genes of toxin A (TcdA) and B (TcdB
), whereas 17/37 (45.9%) of isolates were negative for toxin A and B genes.
Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (toxin A), and tcdB
(toxin B) genes for toxigenic culture of Clostridium difficile.
Testelin et al., "Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (toxin A), and tcdB
(toxin B) genes for toxigenic culture of Clostridium difficile," Journal of Clinical Microbiology, vol.
Clostridium difficile is a gram-positive, anaerobic, sporeforming, rod-shaped bacterium that can produce at least three toxins including two Rho GTPase-glucosylating toxins (TcdA and TcdB
) and the binary C.
The Transparent Cost Database (TCDB
) provides technology cost estimates that can be used to benchmark company costs, model energy scenarios, and inform research and development decisions.
, and a glutamate dehydrogenase detection assay to cytotoxin testing and cytotoxigenic culture methods.
difficile toxins A (TcdA) and B (TcdB
), as well as the CD-specific antigen glutamate dehydrogenase (GDH).
difficile infection including highly sensitive PCR assays that target the toxin genes (mainly tcdB
and tcdC) and the use of algorithms that utilise two or more diagnostic techniques (14,18,19).
Jamshid Tanha and his NRC team have done just that, developing a suite of recombinant antibodies, called single-domain antibodies, capable of binding to TcdA and TcdB
, two toxins produced by E.
The hospital received invaluable assistance in validation of an assay for detection of the Clostridium difficile tcdB
gene and the student acquired an interesting research project that resulted in a thesis, a Masters level degree and a job.
difficile produces two toxins-toxin A (TcdA) and toxin B (TcdB
) -- which cause damage to intestinal cells by binding to carbohydrates on the cell surface and disrupting cell functions such as adhesion.