RT-qPCR was used to quantify the mRNA expression of ALP, Versican, WNT5a, LEF1, [beta] catenin, P cadherin, EpCAM, TCHH, Keratin 75, Keratin 35, and Keratin 32.
Target Forward primer Reverse primer hHPRT ACCAGGTTATGACCTTGATT AAGTTGGCCTAGTTTATGTT ALP GCACCTGCCTTACTAACTCC AACATAGACACTGCCCTCATC Versican TCCAACAGGAAAGGAATGAA TTACTGGGGACAGTGAGGTG WNT5a GGGTGATGCAGATAGGTAGG TCAGGTGTAGGGACAGGAAT LEF1 TGAAGGTGATTCTTGGGTTAT CACGGGCACTTTATTTGACT [beta] Catenin CAGCAGCAATTTGTGGTAGG TAGCTCTTCAGGAAGACGGA P cadherin CACATCTGGGTTAAGGAGTT CAGGAGAAGGCACAGTCGTA EpCAM CAGCGGTTCTTTTGGCATAC TCCCCATTTACTGTCAGGTC EDAR TTGCCTCCTTTCTACTGTTGC GCTTACCTTCCACGACTCCA TCHH CTCCTTGAAAGGGAATTTGG TTCCTTGCTCTGGTCTCCTC Keratin 75 TCAAAGTCAGGTAAGTGGGAGA CAAGATGAAGGTCCTTGTGCT Keratin 35 TGCCCTGACTACCAGTCCTA TCCAAAGCCACTCTGAACCT Keratin 32 CATTTCAGGACCATTGAGGA AGTCCAGTTCCCTTCCCAGA