This set of samples and the reference strains TcIII, TcIV, TcV, and TcVI formed a single cluster that shared a characteristic mitochondrial genome distinct from both TcI and TcII (Figure 2, panel B).
for COII-GPY]) were used: TcI (SilvioX10 cl4, EU302222.1-Silvio10cl1-AY540730.1), TcII (Esmeraldo cl3, AF359035.1-AY540728.1), TcIII (M6241 cl6, AF359032.1- AY484478.1), TcIV (CANIII cl1-AF359030.1), TcV (Mn cl2, DQ343718.1-AY484480.1), and TcVI (CL Brener, DQ343645.1-XM_815802.1).
A new consensus for Trypanosoma cruzi intraspecific nomenclature: second revision meeting recommends TcI to TcVI
. Mem Inst Oswaldo Cruz.
Briones et al., "A new consensus for Trypanosoma cruzi intraspecific nomenclature: second revision meeting recommends TcI to TcVI
," Memorias do Instituto Oswaldo Cruz, vol.
(52) han demostrado que el genotipo de las UDTs TcII, TcV y TcVI
es identico tanto en la madre como en el recien nacido, que es lo esperado cuando se transmite una cepa de T.
Kehewin, Frog Lake, Beaver Lake, Cold Lake, Whitefish Lake and Heart Lake First Nations are member nations of TCVI
TcV and TcVI are largely confined to domestic transmission cycles and are sympatric with severe chronic and congenital human disease in southern South America (2).
Based on nuclear MLST and microsatellite data, all hybrids from Colombia were classified as TcVI, not novel recombinants.
Examination of TcII and TcIII alleles across 5 nuclear loci showed that hybrid haplotypes from Colombia were shared among other TcVI strains from the Southern Cone region of South America and showed negligible affinities to parental alleles from Colombia (online Technical Appendix 1 Figures 1, 2).
Overall, our data support the hypothesis that 2 separate recombination events led to the formation of TcV and TcVI. These interlineage hybrids have distinct nuclear and mitochondrial MLST genotypes and related but independent microsatellite profiles, and most alleles that distinguish between hybrid DTUs (70.4% [38/54 alleles]) were also present in their corresponding parental strains.
TcVI clones from Colombia had more private micro-satellite alleles per locus (0.86) than their southern counterparts (0.43), despite their unequivocal origin in the Southern Cone.
A novel observation among TcVI strains from Colombia was the presence of an anomalous TcV maxicircle.