, a synthetic version of a naturally occurring plant hormone known as a "cytokinin," is not new.
The combination of BAP, TDZ
with ABA was studied for germination of somatic embryos in to plantlets and data were represented in the table-4.
The best TDZ
concentrations were 0.6 [micro]M and 0.8 [micro]M, with both dinducing PLB production from 40% of the leaf segments.
Cotyledon was very recalcitrant, where shoot regeneration was recorded on two culture media only containing 0.2 and 0.4 mg/l TDZ
and one culture media containing 0.4 mg/l TDZ
with 0.1 mg/l IBA with shoot regeneration frequency of 11.11-22.22 (Table 1).
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For growth kinetics 30 days old callus was harvested and 0.1 g callus tissue was inoculated on MS media combined with 4.0 mg/l TDZ
plus 4.0 mg/l NAA.
The product Dropp[R] with 50% of active principle was used as source of TDZ
In order to optimize the best callus induction media leaf sections (0.5-1.0 cm) were cultured on NN medium supplemented with 1.0-3.0 mg L-1 2,4-D combined with 0.5 mg L-1 TDZ
or 1.0 Kinetin (Table 1).
After sterilization, shoot meristem, 1st and 2nd nodal meristem explants were isolated under sterile conditions and incubated on MS (Murashige and Skoog, 1962) medium fortify with 30 g sucrose (Duchefa) per liter, 0.65% agar and 0.05, 0.10, 0.20, 0.40 and 0.80 mg/L Thidiazuron (TDZ
) in Magenta GA7 vessels for in vitro callus formation.