Of 34 isolates resistant to tetracycline, seven isolates harbored more than one resistance gene (1-tetA & tetD, 1-tetA, tetC & tetS, 2-tetC & tetE, 2-tetC & tetS and 1-tetC, tetD & tetE), 4 carried tetA gene and 1 carried tetB gene.
Of 34 tetracycline resistant isolates, seven strains harbored more than one resistance gene (1- tetA & tetD, 1- tetA, tetC & tetS, 2- tetC & tetE, 2- tetC & tetS and 1- tetC, tetD & tetE), 4 carried tetA gene and 1 carried tetB gene.
Of these mechanisms in Gram negative bacteria, an efflux pump system encoded by the genes tetA, tetB, tetC, tetD, tetE and tetG is perhaps the most important (Skockova et al., 2012).
coli isolates recovered from poultry drinking water and the prevalence of tetA, tetB, tetC, tetD, tetE and tetG genes in the local population of E.
Tetracycline resistance genes (tetA, tetB, tetC, tetD, tetE and tetG) were amplified by gradient PCR at a temperature range of 50-60degC using already reported primers (Zhang and Zhang, 2011).
TMTM and IBM, the monosulfides, improve the cure rate to the same extent as TMTD, while TETD appears to be the most effective.
When TMTD, TMTM and TETD are used with the primary accelerator, reversion is aggravated relative to a recipe with no thiuram.
Interestingly enough, both IBM and TMTM generate the same total density, while TBzTD, TETD and IBT are nearly identical.
Table 2 - additives which may produce N-nitrosamines in rubber compounding when decomposed Chemical class MBS, DIBS, OTOS, OTTBS Sulfenamides MTD, TMTM, TETD
, MPTD, DPTT Thiurams ZDMC, ZDEC, ZDBC, Z5MC, ZEPC, Dithiocarbamates ZMPC, TeDMC, CuDMC Sulphur donors DTDM, MBSS
The article shows TBzTD replacing thiuram accelerators such as TMTD and TETD, and CBBS, a delayed action sulfenamide, is compared to MBS, DCBS and other similar accelerators.
A standard EPDM sheeting compound containing TMTD and TETD was evaluated for nitrosamines, with NDMA and NDEA found.
To control these side reactions, various coagents like tetraethyl thiuram disulfide (TETDS
) , heteroatomic ring derivatives , dithiocarbamates [22-24] have been used in recent years.