Of these mechanisms in Gram negative bacteria, an efflux pump system encoded by the genes tetA, tetB, tetC, tetD, tetE and tetG is perhaps the most important (Skockova et al., 2012).
coli isolates recovered from poultry drinking water and the prevalence of tetA, tetB, tetC, tetD, tetE and tetG genes in the local population of E.
Tetracycline resistance genes (tetA, tetB, tetC, tetD, tetE and tetG) were amplified by gradient PCR at a temperature range of 50-60degC using already reported primers (Zhang and Zhang, 2011).
The presence of these non-antimicrobial-drug resistance R plasmid DNA sequences in SGI1 constitutes a molecular signature that firmly establishes the aquaculture origin of the florfenicol resistance and the tetR/ tetG
genes in the S.
Resistance genes were identified by using PCR with primers specific for [bla.sub.TEM] (A), [bla.sub.CARB-2], cmlA, call (C), catIII (C), aadA2, sul1, and tetG
By PCR, all isolates possessed [bla.sub.CRB-2], cmlA, aadA2, sul1, and tetG
but were negative for [bla.sub.TEM], catI, and catIII.
Resistance genetic sequences identified in class I integron-carrying multidrug-resistant strains of Salmonella Integron Serotype sulI pse1 tem oxa2 aadA2 ant(3")-Ia Derby + + Newport + + Paratyphi B + + Saintpaul + + + Sandiego + + Anatum + + Blockley + Brandenburg(a) + + Livingstone + + Ohio + + Ohio + + + Panama + + + Saintpaul + + + Anatum + + Anatum + + Anatum + Blockley + Brandenburg + Brandenburg + Brandenburg + + Bredeney + Derby + + + Heidelberg + + Stanley + Integron pasppflo-like Serotype aac(3)-IA dhfrl tetG (flor) Derby Newport Paratyphi B + + Saintpaul + Sandiego + Anatum Blockley + Brandenburg(a) + Livingstone Ohio + Ohio + Panama + Saintpaul + Anatum + Anatum Anatum Blockley Brandenburg Brandenburg + Brandenburg + Bredeney Derby Heidelberg Stanley + (a) Outbreak strain.
Both tetG and flor resistance determinants, known to characterize MDR-DT104 strains (16), were found in one strain of Paratyphi B isolated from tropical fish imported from Singapore.
Moreover, tetG and flor resistance sequences in one S.