Intestinal trefoil factor, Porcine intestinal epithelial cells, Endotoxin, TFF3, rhTFF3.
Many in vitro studies have shown that TFF3 plays an important role in intestinal diseases.
Since TFF1 and TFF3 are found in the adult brain of mice and rats, it is interesting to investigate their expression in mouse embryonic nervous tissue, and also to screen for their presence in the neurons of the peripheral nervous system both in adult animals and embryos.
Best results were obtained using 1:500 antibody dilution for TFF1 peptide, and 1:5000 for TFF3 peptide.
11 April 2014 - A Chinese study, published today in the journal BMC Gastroenterology, has demonstrated that serum trefoil factor 3 (TFF3) levels may be a better biomarker for gastric cancer than the pepsinogen (PG) test, while combined testing of serum PG and TFF3 could additionally enhance the efficacy of screening for the disease.
According to the data, the serum concentrations of TFF1, TFF2, and TFF3 in the control groups were significantly reduced versus those in the gastric cancer group, excluding TFF2 which was increased in CAG.
Functions of TFF3 may include protecting the mucosa from insults, stabilizing the mucus layer, and effecting healing of the epithelium.
Medullary carcinoma of the colon frequently shows loss of microsatellite instability (MSI) markers, especially MutL homolog 1 (MLH1) and postmeiotic segregation increased 2 (PMS2), and is commonly positive for CDH17, SATB2, calretinin, TFF3, and MUC4.
The eluted TFF3 was again concentrated using centrifugal devices with a 5-kDa cutoff.
Purified TFF3 was subjected to electrophoresis on 15% SDSPAGE according to the method of Laemmli .
The potential role of TFF3
in the gastrointestinal tract has led to an increasing interest in assays able to measure this peptide in humans.
28, 29) For the 18 MC cases, staining for MSI markers (MLH1, PMS2, mutS homolog 2 (MSH2), mutS homolog 6 (MSH6), CDH17, SATB2, CDX2, CK7, CK20, TFF3
, MUC4, calretinin, P504S, villin, and synaptophysin were performed.