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Rapid growth in the TGGs led to lodging of forage before harvests in 1996; therefore, in 1997 there were four harvests.
In each TGG, the 4.5[degrees]C temperature gradient was created through the control of the unidirectional ventilation rate from inlet to outlet end of the TGG (Horton, 1999).
Carbon dioxide was provided from a supply tank outside the TGG and injected into a predilution distribution system placed approximately 1.2 in inside the inlet end of the TGG.
Abbreviations: ADF, acid detergent fiber; B, baseline temperature; DM, dry matter; IVDOM, in vitro digestible organic matter; NDF, neutral detergent fiber; GM, organic matter; PAR, photosynthetically active radiation; RP, rhizoma peanut; TGG, temperature-gradient greenhouse.
The design of the TGGs was described in detail by Sinclair et al.
Photosynthetically active radiation (PAR) was measured with a calibrated quantum sensor (Model LI-6200, LI-COR, Lincoln, NE) located outside of the TGGs.
Carbon dioxide was provided from a supply tank outside the TGGs and injected into a predilution distribution system placed approximately 1.2 m inside the inlet end of the TGG.
Rapid growth in the TGGs led to lodging of forage before harvests in 1996, therefore in 1997 and 1998 a four-cut system was used.
The methodology to control temperature and [CO.sub.2] was based on the TGG infrastructure used by Okada et al.
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