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From the studied trace elements, only Fe demonstrated a significant positive correlation with the MDA level (r=0.666, p=0.018) and a negative significant correlation with the tGSH level (r=-0.689, p=0.013).
Our findings showed a significant positive correlation between the iron concentration in the samples and the lipid peroxidation, as well as a negative correlation between iron concentration and the tGSH level.
The amount of tGSH in the total homogenate was measured according to the method of Sedlak and Lindsay with some modifications (SEDLAK & LINDSAY, 1968).
As shown in table (2), C[Cl.sub.4] intoxication significantly increased MDA and oxGSH formation, lowered serum levels of tGSH and rGSH, and lowered hepatic GR and serum PON1 activities; however, the intake of AGE in combination with C[Cl.sub.4] significantly lowered MDA formation, alleviated GSH depletion, and enhanced GR and PON1 activities (P< 0.01, as compared with C[Cl.sub.4]-treated group).
In the present study, the tGSH level was significantly higher in the RU and RF group compared to the GT.
Table 3 shows the changes in haemoglobin, haematocrit, plasma volume, GSSG, GSH and tGSH in whole blood and RBCs following the competition.
The highest CVs were observed for tGSH using both methods (>12%).
Measurements of total glutathione concentration (tGSH, GSH + GSSG), peroxidase activity (POD), photosynthesis and transpiration were made on plants exposed to different levels of mercuric chloride and salinity (as NaCl).
The hemoglobin (Hb) concentration was determined with a hemocytometer, and 100 [micro]L of the blood was immediately mixed with 12 [micro]L of 10 mmol/L phosphate buffer, pH 7.2 (for tGSH and GSH) or with 12 [micro]L of 0.1 mol/L N-ethylmaleimide (NEM; for GSSG).
Abbreviations tGSH: Total glutathione GSSG: Oxidized glutathione MDA: Malondialdehyde GSH-Px: Glutathione peroxidase HCV: Hepatitis C virus HIV-1: Human immunodeficiency virus-1 FBDs: Former blood donors DCs: Dendritic cells NRTIs: Nucleoside reverse transcriptase inhibitors NNRTIs: Nonnucleoside reverse transcriptase inhibitors ALT: Alanine aminotransferase AST: Aspartate aminotransferase [gamma]-GT: [gamma]-Glutamyl transpeptidase APRI: Aspartate aminotransferase to platelet ratio index FIB-4: Fibrosis index based on four factors ESLD: End-stage liver disease HAART: Highly active antiretroviral therapy.
Similar patterns were shown for tCysGly and tGSH (Table 2) and rCysGly and rGSH (Table 3).
The within-day imprecision (CV; n = 6) measured with a plasma pool was 2.2% for tCys, 1.7% for tCysGly, 1.9% for tHcy, and 7.2% for tGSH at concentrations of 178.0, 27.8, 7.9, and 3.4 [micro]mol/L, respectively.
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- TGS Systems
- TGT LOC