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* A further 15 MAN TGX semitrailer tractors will join the fleet over the course of 2019.
The highest number of 7 branches per plant was found in TGX 1440-1E, and this was followed by 6 branches per plant in TGX 1830-20E, TGX 1910-14F, TGX 1954-1F, TGX 1937-1F, TGX 1904-6F, TGX 1910-13F and TGX 1908-8F.
TGx has drawn wide attention as an approach to study the underlying molecular mechanisms of toxicity since it allows the generation of large and highly informative data sets that lend themselves to bioinformatic analyses (Nuwaysir et al., 1999; Aardema and MacGregor, 2002).
Samples were loaded on the Mini-PROTEAN TGX 4-15% gradient gels (Bio-Rad).
Proteins were separated on 4-20% SDS-PAGE Mini-PROTEAN TGX Precast Gels using a Mini-PROTEAN II apparatus (Bio-Rad Laboratories) and electrophoretically transferred to the nitrocellulose membrane (Hybond-C; GE Healthcare).
After isoelectric focusing, the strips were washed with buffer I (6 M Urea, 375 mM Tris-HCl pH 8.8, 2% SDS, 20% glycerol, 2% (w/v) DTT) for 30 min and then with buffer II (6 M Urea, 375 mM Tris-HCl pH 8.8, 2% SDS, 20% glycerol, 2.5% iodoacetamide (w/v)) for 30 min at room temperature in the dark and subjected to SDS-PAGE using TGX precast gels in a Dodeca gel running system (Bio-Rad, USA).
Similar belt-tightening is happening in Ingolstadt, where VW's Audi brand is based, OsnabrE-ck and Salzgitter, both of which have facilities that respectively produce VW's Tiguan SUV and truckmaker MAN's TGS and TGX heavy trucks.
(U.S.), TGX Medical Systems (U.S.), Vizbee RFID Solutions (France), and Xerafy (Hong Kong).
The Kpr47b Western blot assay was performed as described (5) with the following modifications: Kpr47 antigen was loaded in a Mini-PROTEAN TGX Precast Gel and separated in a Mini Tetra cell electrophoresis module (both from Bio-Rad, Hercules, CA, USA).
Treated samples (20 [micro]g protein per lane) were separated with SDS-PAGE using Mini-PROTEAN TGX Precast Gels (12%, Bio-Rad Laboratories, Inc.).
Briefly, 10 [micro]L of each sample including protein marker (BioRad Precision Plus Protein Western C Standard, Cat#161-0376) were loaded to Western blot gel (BioRad Mini-PROTEAN TGX Stain-Free Gels, 4-15%, 15-well comb, 15 [micro]L, Cat#456-8096) in BioRad 1X Tris/Glycerine/SDS running buffer and run at 250 V for 20 minutes.
Two years later, two models were presented to succeed the TGA in the heavy series: The TGX was designed for long-distance transport while the TGS was used for applications requiring traction and heavy distribution traffic.
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