Developed by THDF in 2009, the Sustainable Cities Institute provides a one-stop shop for cities and sustainability professionals to find vetted best practices from across the country to help them identify and implement local sustainability programs and policies, as well as communicate with other cities about related issues and topics.
Since 2009, NLC's work on sustainability--including a series of topical guides, case studies and a national survey--has been largely supported through THDF as NLC's official Sustainability Partner.
Going forward, NLC and THDF will finalize details of the transition, which is expected to take place in early 2012.
PCR results were positive for the left junction between the thdF
gene of the chromosome and the int gene of Salmonella genomic island 1 (4).
In serovars Agona and Paratyphi B, SGI1 has the same chromosomal location as in serovar Typhimurium DT104, except that they lack the retron sequence found downstream of SGI1; thus it is located between the thdf gene and the yidY gene of their chromosomes (6,14).
These data thus indicate that the serovar Albany strain 7205.00 contains SGI1 at the same chromosomal location as in serovars Typhimurium DT104, Agona, or Paratyphi B (i.e., between the thdf and yidY genes) but lacks the retron sequence found in DT104 and other serovar Typhimurium strains (6,7).
This direct repeat appeared to be a duplication of the last 18 bp of the thdf gene.
The antibiotic-resistance genes are clustered in part of a 43-kb genomic island called Salmonella genomic island I (SGI1), between the thdf and int2 genes of the chromosome (6-10).
These data indicate that the serotype Paratyphi B strain contains SGI1 at the same chromosomal location as in serotype Typhimurium DT 104 or serotype Agona, that is, between the thdf and yidY genes, but lacks the retron sequence found in DT 104 strains and other serotype Typhimurium strains (7,8).