Niedergang, ""Phagosome closure assay" to visualize phagosome formation in three dimensions using total internal reflection fluorescent microscopy (TIRFM
)," Journal of Visualized Experiments, vol.
To take advantage of its excellent features, we combine it with the TIRFM system to enable the monitoring of the interaction between the nanoparticle and the cell membrane at a located region.
The Olympus IX71 inverted microscope with an optical fiber-based TIRFM accessory is used for light excitation.
The images captured in WF and TIRFM by the EMCCD camera are compared in Fig.
Other instrumentation available to Salk researchers includes, but is not limited to, live-cell confocal and fluorescence microscopy solutions from Carl Zeiss Microimaging, high-speed fluorescence and two-photon microscopy from Olympus America, total internal reflection microscopy (TIRFM
) from Nikon Instruments and automation and cell identification technology from Metasystems International.