Niedergang, ""Phagosome closure assay" to visualize phagosome formation in three dimensions using total internal reflection fluorescent microscopy (TIRFM
)," Journal of Visualized Experiments, vol.
Object of the contract is to acquire an optical microscope system by total internal reflection fluorescence (TIRFM
To take advantage of its excellent features, we combine it with the TIRFM system to enable the monitoring of the interaction between the nanoparticle and the cell membrane at a located region.
The Olympus IX71 inverted microscope with an optical fiber-based TIRFM accessory is used for light excitation.
The images captured in WF and TIRFM by the EMCCD camera are compared in Fig.
Total internal reflection fluorescence microscope (TIRFM
), which enables a simultaneous multi-colored lighting and a perfect control of the penetration depth of the evanescent field, regardless of the wavelength used.
Other instrumentation available to Salk researchers includes, but is not limited to, live-cell confocal and fluorescence microscopy solutions from Carl Zeiss Microimaging, high-speed fluorescence and two-photon microscopy from Olympus America, total internal reflection microscopy (TIRFM
) from Nikon Instruments and automation and cell identification technology from Metasystems International.
Contract notice: Total internal reflection fluorescence microscope (tirfm)
Are expected to offer a total reflection fluorescence microscope (TIRFM), which enables simultaneous multi-colored lighting and a perfect control of the penetration depth of the evanescent field, regardless of the wavelength used.: General Facilities: Inverted high-end motorized tripod, fully: Laser safety device: Epifluoreszente lighting from a monochromator: Objective, highly corrected, 10x, 20x, 40x: DualView for simultaneous multi-color recording: Ultra-sensitive digital monochrome back-thinned EMCCD camera: Air Chamber to work at 37 A C, and controlled O2/CO2 environment: Anti-vibration table.