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This solution was maintained at 45[degrees]C for 1h, and then buffered with 0.5 ml of 1M sodium hydroxide and 1 ml of TISAB II.
Therefore, any undetected concentration measurement of a highly diluted sample was regarded to be below 0.02 mg/l and thus it was below the detection limit (BDL) of the instrument and for the same purpose similar measurements in double distilled water used for rinsing apparatus, its cleaners and TISAB were all considered as fluoride-free matrices.
Abbreviations AI: Adequate Intake BT: Black tea CPF: Chlorpyrifos DRVs: Dietary reference values EU: European Union EFSA: European Food Safety Authority F: Fluoride GT: Green tea NRC: National Research Council NZ: New Zealand RoI: Republic of Ireland RME: Reasonable maximum exposure SCHER: Scientific Committee on Health and Environmental Risks TISAB: Total ionic strength adjustment buffer UK: United Kingdom UL: Tolerable Upper Intake Level USA: United States of America WHO: World Health Organization.
* TISAB Blank: Dilute TISAB II with deionized water 1:1
TISAB III was added to the standards to stabilize the ionic strength .
For determination of fluoride ion concentration in saliva, the samples were diluted in total ionic strength adjustment buffer (TISAB II, Orion, Thermo Electron Corporation, Beverly, USA) at a ratio of 1:1.
The fluoride concentration was analysed using a combined electrode Hach and TISAB III, pH 5 (containing 20g NaOH/I as a buffer).
Analysis of fluorine is carried out by combustion of a weighed sample in a silica oxygen flask over an accurately measured volume of water and TISAB buffer solution A fluoride ion selective electrode is used to directly measure the potential of a range of fluoride solutions.
Para que as solucoes-padrao e as amostras sejam de forcas ionicas comparaveis, e adicionado as solucoes um tampao ajustador de forca ionica (Total Ionic Strength Adjustor Buffer III, TISAB III), evitando a reacao desses com o ion fluoreto.
Another factor that might affect the concentration of fluoride in some of these samples is if hydroxide ion was present in any of the reagents used in the analysis though this was taken care of with the use of TISAB solution.
Fluorine concentration in the filtrate was measured after the addition of a total ionic strength adjusting buffer (TISAB IV, Orion Research Inc.) using a Fion selective electrode (Frankenberger et al.
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