1: Reference substances for the development and evaluation of T cell-based assays Substance name CAS# Human Potency Category (1) 2,4-Dinitrochlorobenzene (DNCB) 97-00-7 1 Trinitrobenzene sulfonic acid (TNBS
) 2508-19-2 - 1,4-Phenylenediamine 106-50-3 1 Glyoxal 107-22-2 2 Resorcinol 108-46-3 4 Eugenol 97-53-0 3 Cinnamic alcohol 104-54-1 3 Lactic acid 50-21-5 6 Salicylic acid 69-72-7 6 Glycerol 56-81-5 6 Sodium lauryl sulfate 151-21-3 6 Substance name LLNA Potency Description Category(2) 2,4-Dinitrochlorobenzene (DNCB) Extreme Pos.
(Asteraceae) in TNBS
colitis model," Journal of Ethnopharmacology, vol.
Finally, various carboxyl group-containing MPs were functionalized with ADH (Figure 1) and the amount of hydrazide groups was determined by BCA and verified by the TNBS
Animal pups receive TNBS
acid in 10% ethanol in saline through a catheter inserted 2 cm into the distal colon on postnatal day 10 without anesthesia.
The substitution degrees of amino groups were determined by TNBS
method [24, 25].
Recently, Xu BL showed GQ protected ulcerative colitis induced by TNBS
through attenuating inflammatory and oxidative stress (Xu et ai., 2015).
The chronic intestinal inflammation induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS
) in a mice model of IBD involves production of IL-12 and IFN-[gamma] with subsequent participation of T helper (Th)1 cells (64).
Machida, "Gastrointestinal distribution and absorption behavior of Eudragit-coated chitosanprednisolone conjugate microspheres in rats with TNBS
induced colitis," International Journal of Pharmaceutics, vol.
Use of superoxide dismutase and catalase producing lactic acid bacteria in TNBS
induced Crohn's disease in mice.
O grau de hidrolise foi medido pela metodologia de ADLERNIESSEN (1979) atraves de reacao do TNBS
(acido trinitrobenzeno sulfonico) com a leucina liberada durante a hidrolise, sendo dado como a relacao entre o numero de ligacoes peptidicas clivadas e o numero total de ligacoes peptidicas.