TNMDTotal Niche Market Domination
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When loaded with a 4% stretch, TDSCs highly expressed tenocyte-related markers COL1 and TNMD, while nontenocyte markers LPL, Sox9, Runx2, and Osterix were not affected [9].
SCX: scleraxis; MKX: mohawk; TNMD: tenomodulin; THBS4: thrombospondin-4; TNC: tenascin C; COL1A1: collagen type I; DCN: decorin; ELN: elastin.
Quantitative real-time PCR was performed using SYBR Green PCR master mix (Thermo Fisher Scientific, US) for detecting the expression of ECM genes collagen I (COL I), collagen III (COL III), decorin (DCN), and aggrecan (ACAN); tenogenic markers tenascin-C (TCN), scleraxis (SCX), and tenomodulin (TNMD); osteogenic markers Runt-related transcription factor 2 (RUNX2) and alkaline phosphatase (ALP); chondrogenic markers Sox 9 and collagen II (COL II); and myogenic markers myogenic differentiation antigen (MyoD) and myogenin (MYOG), with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the normalizing gene.