and TCRPa matched each other well from R1 to R5.
Table 1: PCR primers used to detect CagA, CagE, CagM, CagT, orf10, orf13, TnpA
, and TnpB in H.
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Elements belonging to KPC transposon Tn4401, including tnpA
, tnpR, ISKpn6, and ISKpn7 (14), were identified by PCR and sequencing on both KPC-3--encoding plasmids originating from Kpn1 and Eco2.
Sequencing of the insertion element (IS)26-F/R amplification product showed the location of IS26 transposase A gene (tnpA
), followed by a truncated ISEcp1 mobile element upstream of the [bla.sub.CTX-M-15] gene.
Part of an open reading frame that shared 97% nucleotide identity with that of the transposase gene tnpA
of the transposon Tn2501 (Tn3 family) was identified downstream of [bla.sub.IMI-2] (33).