TPMVTomato Planta Macho Viroid
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Shortly after TPMV was isolated, in vitro studies involving primary cultures of guinea pig embryonic kidney, lung, and heart cells supported replication of this virus (13).
To determine the host range of experimental TPMV infection and to ascertain whether susceptibility of small laboratory animals to disseminated TPMV infection is age-dependent, we infected NIH Swiss mice and Mongolian gerbils of different ages, as well as infant deer mice and gray short-tailed opossums (Monodelphis domestica), by the intracerebral route with 6,000 PFU of TPMV (Table 1).
Studies now in progress on experimental TPMV infection in laboratory-reared Asian house shrews should provide information about virus carriage and shedding.
The antigenic relationship of TPMV with 31 other hantavirus isolates has been investigated by ELISA and cross plaque-reduction neutralization tests (PRNTs) by using antisera from experimentally infected animals (9).
Apart from being antigenically distinct, TPMV also appears to be the most genetically divergent member of the Hantavirus genus.
Preliminary studies indicate that TPMV, like PHV, uses [beta]-1 rather than [beta]-3 integrin (I.N.
Alternatively, seroreactivity may indicate infection with a nonrodentborne hantavirus, such as TPMV. Because Asian house shrews are peridomestic, frequently living within or in close proximity to human dwellings, TPMV infection may occur in humans.
To begin to address this issue, researchers collected serum specimens from 363 life-long residents of Mumbai, India, during 1992 and 1993 as part of a study of retroviral infections, and tested them for serologic evidence of TPMV and SEOV infection by using the indirect immunofluorescent antibody technique.
The prototype shrewbome hantavirus, TPMV, must be viewed within this context.
Therefore, it is rather surprising that TGNV did not form a monophyletic group with TPMV. Before this observation becomes either a challenge or support for the hantavirus-host coevolution concept, more extensive sequence data (for comprehensive phylogenetic analysis) and epizootiologic studies (to confirm the natural hosts of both viruses) are necessary.
Its low sequence similarity to other hantaviruses should make this virus serologically distinct from other hantaviruses, as shown for TPMV (10).