To determine the host range of experimental TPMV infection and to ascertain whether susceptibility of small laboratory animals to disseminated TPMV infection is age-dependent, we infected NIH Swiss mice and Mongolian gerbils of different ages, as well as infant deer mice and gray short-tailed opossums (Monodelphis domestica), by the intracerebral route with 6,000 PFU of TPMV (Table 1).
Studies now in progress on experimental TPMV infection in laboratory-reared Asian house shrews should provide information about virus carriage and shedding.
The antigenic relationship of TPMV with 31 other hantavirus isolates has been investigated by ELISA and cross plaque-reduction neutralization tests (PRNTs) by using antisera from experimentally infected animals (9).
Apart from being antigenically distinct, TPMV also appears to be the most genetically divergent member of the Hantavirus genus.
Yanagihara, unpub, data), which suggests that TPMV is nonpathogenic.
Because Asian house shrews are peridomestic, frequently living within or in close proximity to human dwellings, TPMV infection may occur in humans.
To begin to address this issue, researchers collected serum specimens from 363 life-long residents of Mumbai, India, during 1992 and 1993 as part of a study of retroviral infections, and tested them for serologic evidence of TPMV and SEOV infection by using the indirect immunofluorescent antibody technique.
Although limited data do not indicate that TPMV is a human pathogen, other shrewbome hantaviruses may be pathogenic for humans.
In addition, aided by primers based on the complete genome of TPMV, new hantaviruses have been found in 4 shrew species in the family Soricidae from Eurasia and the Americas (J.