5-fold increase in normally healing tibial bone) and impaired mineralization (OC concentrations unchanged) in conjunction with an insufficient increase in osteoclast function (no changes in TRACP 5b activity, no changes in ICTP concentrations after the initial increase until plate osteosynthesis), results similar to those reported by Hermann et al.
We acknowledge TECOmedical, Sissach, Switzerland, for providing the YKL-40 and the TRACP 5b assay.
IDS will supply the TRACP
5b products direct from its UK headquarters with immediate effect, and will begin manufacturing them in-house as soon as possible ( creating a handful of new jobs to add to the existing 50 strong workforce.
The diagnosis of ADO2 (and subsequent classification as an affected individual) was made if there was diffuse osteosclerosis and/or the pathognomonic findings of endobones in any portion of the visualized skeleton and if the individual had diagnostic values for total TRACP and CK-BB, as described previously (18).
MEASUREMENT OF SERUM TRACP 5B AND TOTAL SERUM OSTEOCALCIN
Serum TRACP 5b activity and total serum osteocalcin were measured with in-house immunoassays, as described previously (22, 23).
Similar to total TRACP activity in our earlier study (18), TRACP 5b activity was significantly increased in clinically affected individuals compared with age-matched controls in both age groups (Table 1 and Fig.
Serum TRACP 5b activity was measured with a solid-phase immunofixed enzyme activity assay as described (12), using TRACP 5b purified from human osteoclasts as a calibrator (7).
The clinical sensitivity in BC + was substantially higher for TRACP 5b than for other serum markers of bone resorption (Fig.
The substantially lower clinical sensitivity of NTX and CTX compared with TRACP 5b in BC+ patients suggests that the increased bone resorption in BC+ patients is primarily mediated by matrix metalloproteinases.
Mean TRACP 5b activity was not increased in patients with HF or RF, whereas mean NTX was significantly increased in both diseases (P <0.
EVALUATION OF TRACP
ISOFORMS IN SERUM FROM HEALTHY SUBJECTS