TRE1Tax-Responsive Element 1
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For use as outgroups, a total of 36 insect trehalase genes were obtained from GenBank, including 16 Tre2, 14 Tre1 and another 6 trehalase genes from microbes and mammals (Table 2).
Using the protein sequences, we analyzed the phylogenetic relationships among BdTre2 and other Tre2 and Tre1 genes across insect species based on the neighbor-joining method (Fig.