TRICINE


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AcronymDefinition
TRICINEN-Tris(Hydroxymethyl) methylglycine
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Alternatively, an increased resolution of LPS can be obtained with SDS-PAGE using a Tricine buffer system [53], or by employing polyacrylamide gel concentration gradients within the gel [54,55].
A mixture containing 10 ^L of venom sample (2 mg/ml of crude venom or 1 mg/ml of HPLC fractions), 5 [micro]l of 2X sample buffer (Novex, Invitrogen) and 1 [micro]l of 10X sample reducing agent NUPAGE (Invitrogen[TM], USA) was heated to 100 [grados]C for 5 min and loaded on to a10-20% Tricine acrylamide gels (Invitrogen[TM], USA).
Tricine buffer (pH 80); PEG; sodium chloride; antihuman IgG or IgM antiserum; HEPES buffer (pH 7.4); stabilizers.
Finally, the presence of co-ligands such as tricine and ethylenediamine-N,N'-diacetic acid (EDDA) in the radiolabeling procedure of HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] stabilizes the metal complex (13,14).
SPECs were homogenized in 250 [micro]L of tricine sample loading buffer (BioRad, Hercules, CA) by shearing with progressively smaller gauge needles beginning with 20 g and ending with 26 g.
Buffers used were Tricine for pH 7.0-9.0 and N-cyclohexyl-3-aminopropanesulfonic acid (CAPS) for pH 10.0-11.0.
Novex 10-20% precast tricine gels (Invertogen Inc., Carlsbad, CA) were used.
The apparent molecular weight of the peptide was approximated by tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis.
A stock culture of B.pasteurii is generally maintained in a solid medium containing : 10g trypcase; 5g yeast extract; 4.5g tricine; 5g [(N[H.sub.4]).sub.2]S[O.sub.4]; 2g glutamic acid and final concentration of 1.6% agar, which is autoclaved separately and added after-wards.