The two sweetlips mitochondrial genomes contain 22 tRNA
genes, which are interspersed between rRNA and protein-coding genes, ranging from 67 (tRNACys, tRNASer(AGY)) to 76 (tRNALys(UUR)) nucleotides in length (Table III).
Using blood samples from people with epilepsy, the researchers found that fragment levels of three tRNAs
"spike" in the blood many hours before a seizure.
When cells are stressed, tRNAs
are cut into fragments.
Table 1 Primers, references, and parameters used in polymerase chain reaction (PCR) and Sanger sequencing Amplicon Primer Sequence 5' to 3' PAT-2 CTTTGAAGGCTTTTAGTTTAGATTAAC ECOIB GGTAGTCTGAGTATCGTCG(A/T)G tRNAs
+ COI COIceF ACTGCCCACGCCCTAGTAATGATATTTTTTATGGTNATGCC COIceR TCGTGTGTCTACGTCCATTCCTACTGTRAACATRTG 12Sa ACACATCGCCCGTCACTCTC 16Sb GACGAGAAGACCCTATCGAGC 12S.
fucata martensii encoded 12 PCG, three rRNA genes, and 21 tRNA
genes on the heavy strand (Fig.
Thus there are 20 distinct types of tRNA
molecules, each capable of linking to one particular amino acid.
Rando suspects tRNA
fragments play a central role in both findings, and he is starting experiments now to test that hypothesis.
Existing studies of [m.sup.5]C in cellular RNA have been largely confined to rRNA and tRNA
Muscular mtDNA sequencing showed the heteroplasmic mutations m.5835G>A in MT-TY gene, coding for tRNA
Of these, the GARS gene located on chromosome 7p produces an aminoacyl tRNA
synthetase responsible for charging the associated tRNAs
and the first protein of its kind implicated in any inherited human disorder.
The genome analysis revealed 57 tRNA
and 13 rRNA genes.
Positional isomers of cyclic nucleotides 3',5'-cGMP and 2',3'-cGMP, tRNA
from Torula yeast, and 3',5'-cGMP were purchased from Sigma-Aldrich (Germany).