polyedra transcriptomic data, we identified proteins with high similarity to mammalian TRPM, TRPP, and TRPML.
polyedra, we downloaded from https://www.ncbi.nlm.nih.gov/ more than 370 TRP amino acid (AA) sequences, including members of mammalian TRPA, TRPV, TRPM, TRPC, TRPML, TRPP, Drosophila sp.
The highest similarities and coverage were found with TRPM, TRPP, and TRPML query sequences.
BLASTP analysis identified TRPM, TRPP, and TRPML-like proteins in Lingulodinium polyedra.
The longer AA distance is characteristic for TRPML and TRPP in other organisms (Venkatachalam and Montell, 2007).
BLASTP and phylogenetic inference indicate that Lingulodinium polyedra expresses TRPM (Lp1 and Lp2)-like, TRPP (Lp3-Lp5)-like, and TRPML (Lp6)-like proteins.
TRPP, TRPML, and TRPV have been proposed as the ancestors of metazoan TRP channel subfamilies, based on the presence of these channels and lack of homologs to other metazoan TRP subfamilies in unicellular eukaryotes, including the apusozoan Thecamonas trahens (expressing TRPP and TRPV) and the amoebozoan Dictyostelium (expressing TRPP and TRPML), both located near the base of the unikont branch of the evolutionary tree.
Transient receptor potential (TRP) family has six transmembrane domains that can be further categorized into seven subtypes: TRPV (vanilloid), TRPA (ankyrin), TRPC (canonical), TRPM (melastatin), TRPML (mucolipin), TRPP
(polycystin), and TRPN (no mechanoreceptor potential C) .